Properties and Regulation of Aspartate Kinase from Barley Seedlings (Hordeum vulgare L.).
نویسندگان
چکیده
Aspartate kinase (EC 2.7.2.4) has been purified 8-fold and characterized from germinating barley (Hordeum vulgare) seedlings. The enzyme is inhibited 50% by 0.7 mm l-lysine and almost completely at 5 mm. l-Methionine does not affect the enzyme on its own, but at low concentrations (0.1-1 mm) increases the inhibition in the presence of lysine, indicating that the two amino acids act as cooperative feedback regulators.
منابع مشابه
Lysine Catabolism in Barley (Hordeum vulgare L.).
Lysine catabolism in seedlings of barley (Hordeum vulgare L. var. Emir) was studied by direct injection of the following tracers into the endosperm of the seedlings: aspartic acid-3-(14)C, 2-aminoadipic acid-1-(14)C, saccharopine-(14)C, 2,6-diaminopimelic acid-1-(7)-(14)C, and lysine-1-(14)C. Labeled saccharopine was formed only after the administration of either labeled 2,6-diaminopimelic acid...
متن کاملEndogenous rhythmicity of ethylene production in growing intact cereal seedlings.
Ethylene evolution from etiolated barley (Hordeum vulgare), wheat (Triticum aestivum), and rye (Secale cereale) seedlings during coleoptile growth followed a rhythmic pattern, with a period of about 16 h for barley and wheat and 12 h for rye seedlings. Leaf emergence disturbed the established rhythm of ethylene evolution.
متن کاملImpacts of PEG-6000 pretreatment for barley (Hordeum vulgare L.) seeds on the effect of their mature embryo in vitro culture and primary investigation on its physiological mechanism.
In this paper, we studied polyetheneglycol (PEG) pretreatment effect on the mature embryo culture in vitro by using barley (Hordeum vulgare L.) seeds. Meanwhile, we analyzed and assayed its mineral element and endogenous hormone level. The experimental results were as follows: (1) PEG-6000 imbibition could obviously slow down the water timecourse absorbed by barley seeds; (2) 10% PEG-6000 treat...
متن کاملIn Vivo Nitrate Reduction in Roots and Shoots of Barley (Hordeum vulgare L.) Seedlings in Light and Darkness.
In vivo NO(3) (-) reduction in roots and shoots of intact barley (Hordeum vulgare L. var Numar) seedlings was estimated in light and darkness. Seedlings were placed in darkness for 24 hours to make them carbohydrate-deficient. During darkness, the leaves lost 75% of their soluble carbohydrates, whereas the roots lost only 15%. Detached leaves from these plants reduced only 7% of the NO(3) (-) a...
متن کاملGreen synthesis of Se nanoparticles and its effect on salt tolerance of barley plants
In this study, selenite ions were reduced to selenium nanoparticles using a leaf extract of barley (Hordeum vulgare L.) plants. Characterization of synthesized nanoparticles using Scanning Electron Microscopy (SEM) and UV-visible spectrophotometry indicated the formation of variable size of selenium nanoparticles, suggesting that leaf extract could form polydispersed nanoparti...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Plant physiology
دوره 59 1 شماره
صفحات -
تاریخ انتشار 1977